INDUCTION OF OVARIAN DEVELOPMENT IN AUTOGENOUSAEDES ATROPALPUSBY JUVENILE HORMONE AND 20-HYDROXYECDYSONE
Autor: | Thomas Kelly, Morton S. Fuchs, Suk-Hee Kang |
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Rok vydání: | 1981 |
Předmět: |
Aedes
medicine.medical_specialty 20-Hydroxyecdysone Biology Oocyte biology.organism_classification chemistry.chemical_compound medicine.anatomical_structure Endocrinology chemistry Internal medicine Juvenile hormone medicine Aedes atropalpus Animal Science and Zoology Vitellogenesis Polyacrylamide gel electrophoresis Developmental Biology |
Zdroj: | International Journal of Invertebrate Reproduction. 3:101-112 |
ISSN: | 0165-1269 |
Popis: | Aedes atropalpus is an autogenous mosquito characterized by a first gonadotropic cycle which results in approximately 200 mature oocytes without a bloodmeal. Ovarian development is completely inhibited if these animals are decapitated or ligated between the thorax and abdomen shortly after adult emergence. Injection of 4.8 ng of 20-hydro- xyecdysone into decapitated females 12 h after eclosion restores ovarian development in all females so treated. However, the same amount of 20-hydroxyecdysone injected into isolated abdomens obtained shortly after adult emergence had no discernible effect on vitellogenesis. In contrast, all abdomens which received 0.5 ng of topically applied JH I followed by the injection of 4.8 ng 20-hydroxyecdysone produced mature oocytes. Isolated abdomens were also capable of oocyte maturation when treated with excess amounts of JH alone; JH I was the most effective followed by JH II and then JH III. Polyacrylamide gel electrophoretic analysis of vitellin extracted from the ovaries o... |
Databáze: | OpenAIRE |
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