The effect of pyruvic acid thiosemicarbazone on ornithine carbamoyl transferase ofLycopersicon esculentum Mill

Autor:	J. Miersch, Krauss Gj, Konstantin Grancharov, F. Bublitz, Nadejda Spassovska, Evgeny Golovinsky
Rok vydání:	1986
Předmět:	chemistry.chemical_classification Chemistry Plant Science Horticulture Ornithine In vitro chemistry.chemical_compound Enzyme Biochemistry Ornithine Carbamoyltransferase Citrulline Transferase Pyruvic acid Semicarbazone
Zdroj:	Biologia Plantarum. 28:174-179
ISSN:	1573-8264 0006-3134
DOI:	10.1007/bf02894593
Popis:	The pyruvic acid thiosemicarbazone was found to inhibitin vitro the soluble ornithine carbamoyl transferase as well as the enzyme in a mitochondria-rich cell fraction. For proving the inhibition of ornithine carbamoyl transferase in mitochondria-rich fraction a new method for DEAE-thin-layer chromatography of radiocarbon labelled citrulline and ornithine was used. The mitochondria prepared by discontinuous sucrose density gradient centrifugation are physiologically active and oxidized succinate under state 3 conditions. The respiratory control ratio was determined to 3.3. The results obtained show that this analogue of pyruvic acid is an inhibitor of ornithine carbamoyl transferase.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d7ec1070e399117adfc7e066cd458aa8 https://doi.org/10.1007/bf02894593 Zobrazit plný text záznamu Full text from SpringerLink