| Popis: |
A small plasmid pPA21SG with a size of 6.2 kb was isolated from chlorophenoxyacetic acids-degrading strain Pseudomonas aeruginosa 21SG. The chromosomal location of the 4-CPA, 2,4-D and 2,4,5-T degradation genes was determined by the elimination of plasmid from 21SG strain with ethidium bromide (500 μg/ml). A PCRbased analysis revealed the absence in genome of 21SG strain the tfdA and tftA genes, that responsible for the initiation steps of 2,4-D and 2,4,5-T degradation by strains Cupriavidus necator JMP134 (pJP4) и Burkholderia phenoliruptrix АС1100. |
