IN VITRO ANTIMICROBIAL ACTIVITY AND MIC OF THE EXTRACELLULAR ETHYL ACETATE CRUDE EXTRACT OF ENDOPHYTIC FUNGI FUSARIUM SP. ISOLATED FROM TEPHROSIA PURPUREA ROOT
| Autor: | Deependra Thakur, Kamana Sahani |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Pharmacology Fusarium 030102 biochemistry & molecular biology biology Traditional medicine Pharmaceutical Science Resazurin Bacillus subtilis biology.organism_classification Antimicrobial Tephrosia purpurea Plant use of endophytic fungi in defense 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Agar diffusion test Bacteria |
| Zdroj: | International Journal of Pharmacy and Pharmaceutical Sciences. :48-53 |
| ISSN: | 0975-1491 2656-0097 |
| DOI: | 10.22159/ijpps.2019v11i3.28230 |
| Popis: | Objective: The objective of the present investigation was to find antimicrobial and MIC of endophytic fungi Fusarium sp. isolated from Tephrosia purpurea root. Methods: Well diffusion assay was performed to find out the antimicrobial activity and Resazurin dye reduction method was performed to find out MIC of the extract. Result: The extract showed the highest zone of inhibition of 22.66±0.57 mm, (Bacillus subtilis, MTCC-441) for Gram-positive bacteria and 20.66±0.57 mm, (E. coli, MTCC-443) for Gram-negative bacteria. Furthermore, the MIC of the extract was found to be (31.25 µg/ml-125 µg/ml). Conclusion: Hence, the endophytic fungi isolated from the Tephrosia purpurea root, i.e. Fusarium sp. showed good antimicrobial activity and hence can be used to find a novel drug. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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