Integrated micro/nano-fluidic system for mixing and preconcentration of dissolved proteins

Autor:	Khalid Anwar, Taeheon Han, Sun Min Kim, Samuel Yu
Rok vydání:	2011
Předmět:	Electrokinetic phenomena chemistry.chemical_compound Polydimethylsiloxane chemistry Microfluidics Electrical breakdown Mixing (process engineering) Micromixer Sample preparation Nanotechnology Fluidics Analytical Chemistry
Zdroj:	Microchimica Acta. 173:331-335
ISSN:	1436-5073 0026-3672
Popis:	An integrated micro/nano-fluidic system is presented for protein analysis. It is comprised of an integrated micromixer (IMM) and a preconcentrator with a separation column. The passive and planar type of IMM is based on an unbalanced split and the cross collision of the fluidic streams. The IMM can be easily fabricated and integrated to the microfluidic system. The preconcentrator has nanochannels formed by the electrical breakdown of polydimethylsiloxane (PDMS) membrane by applying a high electrical shock, but without any nano-lithography. The integrated microdevice was used for sample preparation (mixing with tagging molecules) and subsequent concentration of proteins. Proteins were electrokinetically trapped near the junction of the micro/nanochannels. We show a conceptual design and a simple microfluidic system for purposes of mixing and preconcentration.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d5c05d84de836fca68d637ff090ec4e7 https://doi.org/10.1007/s00604-011-0567-6 Zobrazit plný text záznamu Plný text ve formátu PDF