Development and Validation of a High Performance Liquid Chromatographic Method for Determination of Bimatoprost in Chitosan-Based Ocular Inserts
| Autor: | Juçara R. Franca, Tatiana G. Ribeiro, Rachel Oliveira Castilho, André Augusto Gomes Faraco, Christian Fernandes, Luciana D. Batista |
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| Rok vydání: | 2014 |
| Předmět: |
Intraocular pressure
Chromatography Bimatoprost Biochemistry (medical) Clinical Biochemistry Context (language use) Biochemistry High-performance liquid chromatography Controlled release Analytical Chemistry Chitosan chemistry.chemical_compound Prostaglandin analog chemistry Drug delivery Electrochemistry medicine Spectroscopy medicine.drug |
| Zdroj: | Analytical Letters. 48:531-540 |
| ISSN: | 1532-236X 0003-2719 |
| Popis: | Glaucoma is one of the most prominent causes of blindness around the world. Conventional eye-drops, currently used in the treatment of the disease, must be taken every day and can cause severe systemic side effects. In order to address these problems, a solvent casting method was used to produce chitosan-based polymeric ocular inserts for controlled release of bimatoprost, a prostaglandin analog highly powerful in reducing intraocular pressure. The development and validation of quality control methods are an important concern in the field of pharmaceutical technology when dealing with new drug delivery systems. In this context, a rapid method using reverse phase high-performance liquid chromatography with ultraviolet detection was developed and validated for the determination of the pharmaceutical into the chitosan-based polymeric matrix. The method was linear (determination coefficient higher than 0.99) over the range of 3.00–15.00 µg/mL, precise (relative standard deviation lower than 5%), accurate (rec... |
| Databáze: | OpenAIRE |
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