Effectiveness of three instrumentation systems to remove Enterococcus faecalis from root canals
| Autor: | Sandra Márcia Habitante, M. Matos Neto, Antonio Olavo Cardoso Jorge, Jane Rose Dias Dionisio Rodrigues, Silvana Soléo Ferreira dos Santos, Mariella Vieira Pereira Leão |
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| Rok vydání: | 2011 |
| Předmět: |
Dental Pulp Cavity
food.ingredient biology business.industry Root canal Significant difference Dentistry Sterilization (microbiology) biology.organism_classification Enterococcus faecalis chemistry.chemical_compound medicine.anatomical_structure food chemistry Sodium hypochlorite medicine Brain heart infusion Agar business General Dentistry |
| Zdroj: | International Endodontic Journal. 45:435-438 |
| ISSN: | 0143-2885 |
| DOI: | 10.1111/j.1365-2591.2011.01994.x |
| Popis: | Aim To assess the effectiveness of three systems of mechanical preparation to reduce Enterococcus faecalis within root canals. Methodology Twenty-four human single-rooted canine teeth were standardized to a length of 17 mm and the canal contents removed using a size 20 K-file, as the last apical file. After irrigation and sterilization, the canals were contaminated with E. faecalis and incubated for 21 days at 37 � C with 5% CO2. Then, the teeth were divided into three groups for mechanical preparation with: ProTaper rotary system, ProTaper manual system and manual K-files. Samples of the root canal contents, before and after the debridement, were collected with sterile paper points for 1 min. Then, the samples were diluted and plated in Brain Heart Infusion (BHI) agar. The colony-forming units were counted and the percentage reduction calculated. The reduction and log CFU mL )1 were compared between groups using Wilcoxon nonparametric test and two-way analysis of variance, respectively. Results There was a significant reduction in the number of CFU/mL (P = 0.000) before and after debridement for all the systems used. However, there was no significant difference between the systems. Conclusion All the three instrumentation systems reduced E. faecalis counts to a similar degree. |
| Databáze: | OpenAIRE |
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