| Popis: |
A total of (213) canned food samples comprising of Tuna &Sardines,Juices, Tomatoes pasts, Jam and Beef were randomly collected fromsuper stores and local markets in Tanta city from Awlad Ragab,Fatthallah, Munshwi, Casion. Also 24 fast food samples collected fromlocal cafeterias and restaurants in Tanta city from Al Gaan, Abu Deshish,Al Baraka, Abu Owaf. All canned food samples were within expiry date,none of which is bloated, leaking and/or physically damaged. Sampleswere investigated for some bacteria and fungi using specific media andincubated for suitable incubation period. The results revealed thatspecies of microbes isolated in this study namely Bacillus subtilis,Bacillus cereus, Bacillus atrophaeus., Staphylococcus saprophyticus,Enterococcus faecalis, Staphylococcus epidermides, E. coli, Klebsiella,Salmonella , Aspergillus niger, Penicillium notatum, Candida tropicalisand Saccharomyces cerevisiae. Biochemical tests were performed for allisolates to know the most common isolates (Bacillus cereus andAspergillus niger). Antimicrobial activities of chitosan were investigatedagainst the most common isolates B. cereus and A. niger. Resultsrevealed that chitosan has antibacterial activity towards Bacillus cereus.The minimum inhibition concentration (MIC) for chitosan was 6.25μg/ml with mean diameter of inhibition zone 8 mm. Also chitosan hasantifungal activity toward Aspergillus.niger with minimum inhibitionconcentration (MIC) 30mg/ml and percent of fungal growth inhibition16.7%. |
