Polyamino acid-based gemcitabine nanocarriers for targeted intracellular drug delivery
| Autor: | Zhihui Qin, Haijie Han, Wenzhuo Teng, Fan Jia, Jian Ji, Qiao Jin |
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| Rok vydání: | 2017 |
| Předmět: |
endocrine system diseases
Polymers and Plastics Biocompatibility Organic Chemistry Bioengineering 02 engineering and technology Receptor-mediated endocytosis Conjugated system 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Biochemistry Combinatorial chemistry Micelle 0104 chemical sciences chemistry.chemical_compound chemistry Copolymer Organic chemistry Nanocarriers 0210 nano-technology Cytotoxicity Ethylene glycol |
| Zdroj: | Polymer Chemistry. 8:2490-2498 |
| ISSN: | 1759-9962 1759-9954 |
| DOI: | 10.1039/c7py00443e |
| Popis: | In the present study, we have successfully fabricated a biocompatible polyamino acid-based nanocarrier with reduction-sensitivity and targeting ability for gemcitabine (GEM) delivery. GEM-conjugated polyamino acid biotin-poly(ethylene glycol)-block-poly[(L-lysine-co-L-leucine)-graft-(GEM-co-rhodamine B)] (Biotin-PEG-b-P[(Lys-co-Leu)-graft-(GEM-co-Rho B)]) was synthesized by ring-opening copolymerization of L-lysine-N-carboxyanhydride (Lys-NCA) and L-leucine-N-carboxyanhydride (Leu-NCA) using biotin-poly(ethylene glycol)-amine (Biotin-PEG-NH2) as an initiator, and then disulfide linked GEM derivatives (HSEA-GEM) and Rhodamine B isothiocyanate (Rho B) were grafted onto polyamino acids. The obtained GEM-conjugated polyamino acids could self-assemble into nano-sized micelles in aqueous solution with a uniform spherical shape. The GEM nanocarriers showed reduction-sensitive drug release. Cell culture demonstrated that the polyamino acid itself showed excellent biocompatibility but high cytotoxicity when conjugated with GEM. What's more, the biotin-conjugated polyamino acid-based micelles could induce a remarkably higher internalization via receptor mediated endocytosis than non-biotin micelles. Hence, such biocompatible GEM-conjugated polyamino acids might provide a potential strategy for cancer therapy. |
| Databáze: | OpenAIRE |
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