Hybrid near-field scanning optical microscopy tips for live cell measurements
| Autor: | Jeffrey R. Krogmeier, Jonathan Carnell, Luka K. Kapkiai, Robert C. Dunn, David Moore-Nichols |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Atomic force microscopy cantilever
Optical fiber Materials science Physics and Astronomy (miscellaneous) business.industry Latex Spheres Fluorescence law.invention Optics Optical microscope law Scanning ion-conductance microscopy Near-field scanning optical microscope business Arterial smooth muscle cells |
| Zdroj: | Applied Physics Letters. 84:3750-3752 |
| ISSN: | 1077-3118 0003-6951 |
| DOI: | 10.1063/1.1737464 |
| Popis: | We report a near-field scanning optical microscopy (NSOM) probe that enables high-resolution imaging of living cells under physiological buffered conditions. The hybrid design combines a conventional fiber optic near-field probe with a standard atomic force microscopy cantilever. Imaging of fluorescent latex spheres suspended in an acetate matrix demonstrates the subdiffraction limited fluorescence and topography capabilities of the tips. The reduced spring constant of the hybrid tip is also shown to be amenable to measurements on living cells. NSOM fluorescence and topography measurements on living human arterial smooth muscle cells under buffered conditions are demonstrated. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|