| Popis: |
Three DNA sequences specific for the causal agent of bacterial ring rot of potatoes, Clavibacter michiganense subsp. sepedonicum, have been isolated from a library prepared from total DNA. One of the DNA probes is a PstI fragment of 2.6 kilobases (kb), which contains a region that is repeated numerous times. By using a probe labeled with 32 P-dCTP, 0.5 ng of total DNA could be detected. The same detection limit was obtained with a biotinylated probe, whereas a sulphonated probe was only about one-third as sensitive |
