Influence of exogenous NO donator and variations in the Ca2+ content on transport activity related to tonoplast proton pumps in ontogenesis and under hyperosmotic stress

Autor: E. V. Kolesnikova, L. V. Dudareva, L. I. Donskaya, R. K. Salyaev, V. N. Nurminsky, N. V. Ozolina, I. S. Nesterkina
Rok vydání: 2010
Předmět:
Zdroj: Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology. 4:297-301
ISSN: 1990-7494
1990-7478
Popis: The changes in transport activity of tonoplast proton pumps under the influence of exogenous NO donator and modulation of Ca2+ concentration jointly and separately were investigated at different stages of ontogenesis and under hyperosmotic stress. The results suggest that both exogenous NO donator and Ca2+ ions can influence the activity of transport processes related to tonoplast and this influence is especially evident in the period of growth and accumulation of metabolites. Under hyperosmotic stress, H+-pyrophosphatase plays a more important role than H+-ATPase: the activity of the former increases 2.3-fold compared to the control osmotic conditions, whereas the activity of H+-ATPase is practically unchanged. H+-pyrophosphatase was more responsive to the presence of exogenous NO donator and to variations in Ca2+ concentration. The effects of exogenous NO donator on tonoplast proton pumps depended on the concentration of Ca2+, which apparently can mediate NO action.
Databáze: OpenAIRE
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