Investigation of the complex antibiotic INA-5812
| Autor: | Roman S. Borisov, Igor A. Prokhorenko, V. V. Pogozheva, E. A. Rogozhin, Elena G. Gladkikh, L. G. Stoyanova, Andrey A. Formanovsky, Valeriya V. Kulyaeva, M. A. Sukonnikov, O. A. Lapchinskaya, Anton P. Tyurin, Vladimir A. Brylev, Ludmila A. Baratova, Marina V. Serebryakova, Vera A. Alferova, A. P. Topolyan, G. S. Katrukha, Alexander M. Korolev, P. V. Coodan, Maxim V. Shuvalov, Vadim N. Tashlitsky, Alexander L. Ksenofontov, A. V. Timofeeva, M. S. Slyundina, Ilya A. Osterman |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Tris Chromatography biology medicine.drug_class Organic Chemistry Antibiotics Fractionation biology.organism_classification Antimicrobial Biochemistry Streptomyces High-performance liquid chromatography Glycopeptide 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry medicine Derivatization |
| Zdroj: | Russian Journal of Bioorganic Chemistry. 42:664-671 |
| ISSN: | 1608-330X 1068-1620 |
| Popis: | A concentrate with the antimicrobial activity has been isolated from the culture broth of Streptomyces roseoflavus INA-Ac-5812. Its further fractionation by reversed-phase HPLC has resulted in six fractions. It has been established by MALDI-TOF and ESI-MSn precision mass-spectrometry methods that the main components of the complex antibiotic are several closely related compounds, presumably of a glycopeptide nature. The fraction containing an individual component with a mass of 1845.788 Da has been characterized by UV/Vis absorbance and fluorescence spectra, amino acid analysis, and derivatization with tris(2,6-dimethoxyphenyl)methyl cation. The activity of fractions against pathogenic microbes has been studied. The results allow the supposition that the INA-5812 antibiotic complex is a glyco- or lipoglycopeptide antibiotic of a new type, which is very promising for further study. |
| Databáze: | OpenAIRE |
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