| Autor: |
Zhuangzhuang Wu, Shiwei Chen, Zhen Shen, Taiyong Wu, Jian Li, Yi Feng, Long Zhang, Lingan Huang, Li Guo, Xiaochen Qiao, Yuan Li, Zhi Lv |
| Rok vydání: |
2022 |
| DOI: |
10.21203/rs.3.rs-1936030/v1 |
| Popis: |
The emergence of neoadjuvant chemotherapy has improved the prognosis of patients with osteosarcoma (OS), but metastasis and recurrence have become the difficulty of treatment. Necdin (NDN) exists as a tumor suppressor gene in some tumors, but its role in osteosarcoma is not clear, while c-Myc is a clear oncogene. This study found that the expression of NDN is low expressed and c-Myc is high expressed in osteosarcoma. In vitro overexpression of NDN can suppress the proliferation and promote apoptosis of osteosarcoma cells and reduce its invasiveness; at the same time, NDN can inhibit the expression of c-Myc, MMP2, and MMP13. It has been confirmed in vivo studies that NDN is a tumor suppressor of osteosarcoma which can effectively suppress tumor growth and lung metastasis in xenograft nude mice. Transcriptomic testing found and western blot confirmed that NDN affects the expression of c-Myc, MMP2, and MMP13 genes by affecting key proteins of the Wnt/β-catenin signaling pathway. Our results show that NDN can not only inhibits tumor growth in osteosarcoma, but also inhibits the lung metastasis of osteosarcoma by affecting Wnt/β-catenin signaling pathways. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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