LASER FLUORESCENCE SPECTROSCOPY AS A METHOD FOR STUDYING HUMIC SUBSTANCE
| Autor: | Victor V. Fadeev, E. M. Filippova, S. M. Glushkov, Tatiana A. Dolenko, V.V. Chubarov |
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| Rok vydání: | 2001 |
| Předmět: | |
| Zdroj: | Applied Spectroscopy Reviews. 36:87-117 |
| ISSN: | 1520-569X 0570-4928 |
| DOI: | 10.1081/asr-100103091 |
| Popis: | This paper summarizes the results obtained by fluorescence spectroscopic techniques with conventional and laser light sources for the characterization of the spectral luminescent properties of aquatic humic substances (AHS). The band profiles of corrected absorption, fluorescence emission, and fluorescence excitation spectra are compared in graphical form with unique functions used for the axes. In order to calculate the quantum efficiency of fluorescence, we used the Raman signal from water molecules as an internal reference. The temperature dependence of the fluorescence spectra of humic matter has been recorded in solid (−160°C to 0°C) and in liquid (0°C to +300°C) aqueous solutions. The behavior of the fluorescence band shape is discussed. A new method of nonlinear fluorimetry is proposed to enhance the capabilities of fluorescence spectroscopy. This method is based on the fluorescence saturation effect manifesting itself under powerful laser pumping (excitation) conditions. The use of the technique a... |
| Databáze: | OpenAIRE |
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