Influence of SIN-1 on Platelet Ca2+ Handling in Patients with Suspected Coronary Artery Disease: Ex Vivo and In Vitro Studies

Autor:	Thuc Do Pham, C. Le Feuvre, Annie Brunet, Marie-Aude Devynck, Vacheron A, Jean-Philippe Metzger, K.-H. Le Quan Sang
Rok vydání:	2000
Předmět:	Thapsigargin biology Superoxide business.industry Hematology Pharmacology Nitric oxide Superoxide dismutase chemistry.chemical_compound Thrombin chemistry In vivo Immunology medicine biology.protein Platelet business Ex vivo medicine.drug
Zdroj:	Thrombosis and Haemostasis. 83:752-758
ISSN:	2567-689X 0340-6245
Popis:	SummaryThe 3-morpholinosydnonimine (SIN-1) generates both nitric oxide (NO) and superoxide anion (O2−). It elicits dose-dependent vasodilation in vivo, in spite of the opposite effects of its breakdown products on vascular tone and platelet aggregation.This study was designed to investigate the influence of intravenous SIN-1 injection on platelet Ca2+ handling in patients undergoing coronary angiography. SIN-1 administration reduced cytosolic [Ca2+] in unstimulated platelets by decreasing Ca2+ influx. It attenuated Ca2+ mobilization from internal stores evoked by thrombin or thapsigargin. In vitro studies were used as an approach to investigate how simultaneous productions of NO and O2− from SIN-1 modify thrombin- or thapsigargin-induced platelet Ca2+ mobilization. Superoxide dismutase, the O2− scavenger, enhanced the capacity of SIN-1 to inhibit Ca2+ mobilization but catalase had no effect.This suggests that the effects of SIN-1 on platelet Ca2+ handling resemble those of NO, but are modulated by simultaneous O2− release, independently of H2O2 formation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d1ad1e7bd087ce5e62d4662ec3b3a0bb https://doi.org/10.1055/s-0037-1613904 Zobrazit plný text záznamu