| Autor: |
Nicholas A. Vickers, Thomas G. Bifano, Ji-Xin Cheng, Hongli Ni, Peng Lin, Yuying Tan, Huate Li |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Advanced Chemical Microscopy for Life Science and Translational Medicine 2021. |
| DOI: |
10.1117/12.2576997 |
| Popis: |
Stimulated Raman scattering (SRS) microscopy enables the imaging of molecular events on a human subject in vivo, such as filtration of topical drugs through the skin and intraoperative cancer detection. A typical approach for volumetric SRS imaging is through piezo scanning of an objective lens, which often disturbs the sample and offers a low axial scan rate. To address these challenges, we have developed a deformable mirror-based remote-focusing SRS microscope, which not only enables high-quality volumetric chemical imaging without mechanical scanning of the objective but also corrects the system aberrations simultaneously. Using the remote-focusing SRS microscope, we performed volumetric chemical imaging of living cells and captured in real time the dynamic diffusion of topical chemicals into human sweat pores. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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