| Popis: |
The purpose of this research is to isolate sasanquasaponin and its hydrolyzed products from the defatted seeds of C. oleifera and evaluate their antioxidative effects in vitro and in vivo. Their structures were analyzed by UV, MS, 1H NMR and 13C NMR spectra. Free radical scavenging activities in vitro were determined by DPPH and ABTS; antioxidative activities in vivo were assessed by MDA, SOD and GSH-Px levels in serum and brain tissue of mice after intragastric administration of the products at 50, 200 mg/kg/d for 30 days. Acid hydrolyzed product and sapogenin had stronger antioxidative activities than the sasanquasaponin. They eliminated DPPH and ABTS radicals, significantly (pp>0.05). On the basis of molecular structure in sasanquasaponin, hydroxyl and tigloyl groups are helpful to free radical scavenging activity; smaller molecules and hydrophobic property are beneficial to their absorption and antioxidative effects in vivo. Acid hydrolyzed product and sapogenin are more valuable to protect body against damage from free radicals. |
