Enhancing the degradation of Aflatoxin B1 by co-cultivation of two fungi strains with the improved production of detoxifying enzymes

Autor:	Jinshui Wang, Dapeng Wu, Yin Haicheng, Shuoye Yang, Yan Shen, Huang Wei, Qipeng Yuan, Yawei Zhao, Le Wang, Liang Wenhui
Rok vydání:	2022
Předmět:	chemistry.chemical_classification Aflatoxin biology Chemistry Chemical structure Aspergillus niger Ultrafiltration General Medicine biology.organism_classification Analytical Chemistry Enzyme Extracellular Degradation (geology) Pleurotus ostreatus Food science Food Science
Zdroj:	Food Chemistry. 371:131092
ISSN:	0308-8146
DOI:	10.1016/j.foodchem.2021.131092
Popis:	After the co-culture of Aspergillus niger and Pleurotus ostreatus, the obtained extracellular crude enzymes solution was employed to aflatoxin B1 (AFB1) degradation. The maximum AFB1 degradation with co-culture reached 93.4%, which increased by 65.9% and 37.6%, respectively, compared with those of the mono-culture of Pleurotus ostreatus and Aspergillus niger. The molecular weight of the key detoxifying enzymes isolated by ultrafiltration was 58 and 63 kDa by SDS-PAGE analysis. The purified detoxifying enzymes had a high detoxification effect on AFB1 with the degradation rate of 94.7%. It was found that the co-culture of Pleurotus ostreatus and Aspergillus niger promoted the production of 58 and 63 kDa detoxifying enzymes to enhance the AFB1 degradation. The chemical structure of major degradation products of AFB1 by the mixed cultures were preliminarily identified by LC-Triple TOF MS. Two pathways of AFB1 degradation were inferred with the high potential of fungal co-cultivations for AFB1 detoxification applications.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d0e9dcb0fd4e9ca8d44b4b66427d1739 https://doi.org/10.1016/j.foodchem.2021.131092 Zobrazit plný text záznamu Full Text from ScienceDirect