Homogeneous enzyme immunoassay for cannabinoids in urine

Autor:	G L Rowley, J K Kam, R Rodgers, C P Crowl, E F Ullman, M W Hu, W M Eimstad, R C Ronald
Rok vydání:	1978
Předmět:	chemistry.chemical_classification Chromatography medicine.diagnostic_test Microgram medicine.medical_treatment Metabolite Biochemistry (medical) Clinical Biochemistry Liter Urine Malate dehydrogenase chemistry.chemical_compound Enzyme Biochemistry chemistry Immunoassay medicine Cannabinoid
Zdroj:	Clinical Chemistry. 24:95-100
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/24.1.95
Popis:	We describe a homogeneous enzyme immunoassay for measurement of cannabinoid metabolites, as well as delta9-tetrahydrocannabinol (I) in urine. Malate dehydrogenase from pig heart mitochondria was labeled with a derivative of I. The compound used to calibrate the assay was the I metabolite, 11-nor-delta9-tetrahydrocannabinol-9-carboxylic acid (II). With 15 microgram of II per liter of urine as the cutoff concentration, the assay can detect 25 microgram of II per liter with greater than 95% confidence. A positive response was obtained for urine specimens assayed within 30 min after exposure to cannabinoids. However, the persistence of metabolites of I in urine indicates that assay of this fluid is useful as an indicator of cannabinoid use but not as an indicator of intoxication.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d0b6043120e3e3640b52c31c4bbe85da https://doi.org/10.1093/clinchem/24.1.95 Zobrazit plný text záznamu