QUANTITATION OF A NEW MACROLIDE IN PLASMA BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY WITH TANDEM MASS SPECTROMETRIC DETECTION: COMPARISON WITH ELECTROCHEMICAL DETECTION
| Autor: | D. J. Daszkowski, S. Menacherry, T. El-Shourbagy, K. C. Marsh, S. L. Carroll, V. S. Ong |
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| Rok vydání: | 1999 |
| Předmět: |
Analyte
Chemical ionization Chromatography Chemistry Clinical Biochemistry Extraction (chemistry) Analytical chemistry Ethyl acetate Pharmaceutical Science Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Liquid–liquid extraction Sample preparation Quantitative analysis (chemistry) |
| Zdroj: | Journal of Liquid Chromatography & Related Technologies. 22:641-657 |
| ISSN: | 1520-572X 1082-6076 |
| Popis: | A method involving high performance liquid chromatography with tandem mass spectrometric detection was developed to determine the concentration of a new macrolide derivative, ABT-229, in human plasma. The analyte was extracted using liquid-liquid extraction with a mixture of ethyl acetate and hexane at basic pH. Detection was highly specific for the analyte of interest, as evidenced by the lack of interference in the chromatograms of drug-free plasma extracts. The lower limit of quantitation was 0.10 ng/mL based on 1 mL of extracted plasma. The linear calibration range of the procedure extended from 0.10 to 199 ng/mL. The method proved reliable and rugged; within-run and day-to-day relative standard deviations were less than 10%. The stability of ABT-229 under frozen storage, room temperature, and freeze/thaw conditions was found to be excellent. Plasma concentrations determined by the method were highly comparable with data obtained by electrochemical detection. |
| Databáze: | OpenAIRE |
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