Short-term fasting in obesity fails to restore the blunted GH responsiveness to GH-releasing hormone alone or combined with arginine

Autor: M. Fakeeh, P.-M. G. Bouloux
Rok vydání: 1995
Předmět:
Zdroj: Clinical Endocrinology. 43:657-664
ISSN: 1365-2265
0300-0664
DOI: 10.1111/j.1365-2265.1995.tb00531.x
Popis: Summary OBJECTIVE Fasting is known to clearly increase both spontaneous and GHRH-stimulated GH secretion in normal subjects and this effect is likely to be due to hypothalamic mechanism(s). Our aim was to clarify the effect of a 3 or 4-day fast, on the GH response to GHRH alone or combined with arginine, an amino acid probably acting via inhibition of hypothalamic somatostatin release. DESIGN Two tests with GHRH (1 μg/kg i.v.), administered either alone or in combination with arginine (ARG, 0.5 g/kg i.v.) were performed, in a randomized order at least 3 days apart. In obese women the two tests were repeated after a 3 or 4-day fast. PATIENTS Seven obese women (06, aged 17–54 years, BMI 42.4 ± 3.6 kg/m2, waist-hip ratio (WHR) 0.85±0.01) and ten healthy women, as control subjects (CS, aged 20–44 years, BMI 23.1 ±1.1 kg/m2, WHR 0.79 ± 0.01) were studied. MEASUREMENTS Serum GH and IGF-I levels were measured by radioimmunoassay. The GH secretory responses were expressed either as absolute values (mU/l) or as areas under the curve (AUC, mU/l/h) calculated by trapezoidal integration. IGF-I concentrations were expressed as absolute values (μg/l) with reference to a pure recombinant IGF-I preparation. Results are expressed as mean ± SEM. RESULTS Basal GH and IGF-l levels in OB were lower than in CS (0.8 ± 0.2 vs 4.8±1.0 mU/I, P
Databáze: OpenAIRE
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