Effectiveness of a detached-leaf assay as a proxy for stem inoculations in backcrossed chestnut (Castanea) blight resistance breeding populations
| Autor: | J. R. McKenna, Nicholas R. LaBonte, Keith E. Woeste |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Canker Ecology Inoculation fungi Castanea dentata food and beverages Forestry Biology biology.organism_classification medicine.disease 01 natural sciences food.food 03 medical and health sciences Horticulture 030104 developmental biology food Chestnut blight Botany medicine Blight 010606 plant biology & botany |
| Zdroj: | Forest Pathology. 47:e12313 |
| ISSN: | 1437-4781 |
| DOI: | 10.1111/efp.12313 |
| Popis: | Summary A recently developed detached-leaf blight resistance assay has generated interest because it could reduce the amount of time needed to evaluate backcrossed hybrid trees in the American chestnut blight resistance breeding programme. We evaluated the leaf inoculation technique on a sample of advanced progeny from the Indiana state chapter American Chestnut Foundation breeding programme, along with susceptible American chestnut (Castanea dentata), the recurrent parent, and resistant Chinese chestnut (Castanea mollissima), the donor parent for blight resistance. In experiments over 2 years using two pathogen isolates, we found no biologically meaningful relationship between leaf lesion size and the size (length and width) or severity (1–5 canker severity rating) of stem cankers on 5-year-old trees. Chinese chestnuts did develop significantly smaller leaf lesions than American or backcrossed chestnuts. We conclude that while the detached-leaf assay may have utility in some chestnut breeding applications, it is not a suitable proxy for the established practice of stem inoculations. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text