| Autor: |
Julie Isbell, Leigh Greer, Carlos Bachier, Haydar Frangoul, Katie Bruce, Misty Evans, Darren Johnson, Jennifer Domm, Sandra Ward |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
Biology of Blood and Marrow Transplantation. 25:S203 |
| ISSN: |
1083-8791 |
| DOI: |
10.1016/j.bbmt.2018.12.276 |
| Popis: |
Background Approximately 40-50% of HSCT are ABO incompatible and bone marrow (BM) products contain 25-35% of incompatible RBCs. RBC reduction can be performed using several different techniques including manual RBC sedimentation using hydroxyethyl starch (HES) or Ficoll-Hypaque density gradient separation with centrifugation or sepax automated device. Volume reduction is often necessary in pediatric patients who cannot tolerate large BM volume. Manual separation can be associated with cell loss of up to 50% of the total nucleated cell dose. Methods We have performed RBC depletion and volume reduction using Optia Spectra following the manufacturer instructions. Each BM unit was processed for 5-10 cycles at 120 ml/minute flow. Results Since 2016 we have processed 22 BM products using this automated system for ABO incompatibility (major mismatch in 10 and minor mismatch in 6) or for volume reduction (n=6). The median age of patients was 23.5 years (range 1 - 62) with 9 patients Conclusion Automated bone marrow processing using the Optia Spectra is efficient in RBC and volume reduction and can result in excellent cell recovery. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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