| Autor: | Hemanshu S. Shah, Bhiku Patel, Pamela Pasciak, Kirk J. Leister, Linda M. Kozick, Cheng-Der Yu, Roger K. Vanderlaan, Elizabeth L Tan |
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| Rok vydání: | 1993 |
| Předmět: |
Pharmacology
Preservative TGF alpha business.industry Organic Chemistry Pharmaceutical Science Antimicrobial Semisolid Dosage Form Dosage form Biotechnology chemistry.chemical_compound medicine.anatomical_structure chemistry medicine Molecular Medicine Pharmacology (medical) Food science Sorbic acid Fibroblast business Transforming growth factor |
| Zdroj: | Pharmaceutical Research. 10:1238-1242 |
| ISSN: | 0724-8741 |
| DOI: | 10.1023/a:1018940907187 |
| Popis: | The selection of an ideal semisolid vehicle for growth factors presents a challenge. Some antimicrobial agents are known to delay wound healing. The objective of this investigation was to identify appropriate preservatives and vehicles for TGF-α. Criteria for acceptance are noninterference with the mitogenic activity of TGF-α as well as adequate product preservation. Vehicles considered were o/w creams, ointments, and a gel. Combinations of six preservatives were tested. Selection was determined using both microbial preservative challenge and TGF-α mitogenic assay. In the former, 10 species of microorganisms were inoculated into formulation samples. At selected time intervals, it was determined whether colonies decreased, increased, or remained constant. In the mitogenic assay, samples of either preservatives or formulation prototypes were introduced to TGF-α-stimulated fibroblast cell cultures. Mitogenesis was determined by measuring 3H-dThd uptake into newly synthesized DNA. As preservatives, sorbic acid and quaternium-15 appear to satisfy both selection criteria. A thermosetting gel appears most promising as vehicle. |
| Databáze: | OpenAIRE |
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