The molecular evolution of prostate cancer cell line exosomes with passage number

Autor:	T. Deng, J. Kimbrough, Teresa T. Tinder, David Spetzler, B. Moran, A. Conrad, Traci Pawlowski, C. Kuslich, J. Kim, P. Esmay
Rok vydání:	2010
Předmět:	Cancer Research Cell type Oncology Membrane protein Cell culture Vesicle microRNA Biology Molecular biology Exosome Exocytosis Microvesicles Cell biology
Zdroj:	Journal of Clinical Oncology. 28:e21071-e21071
ISSN:	1527-7755 0732-183X
DOI:	10.1200/jco.2010.28.15_suppl.e21071
Popis:	e21071 Background: Exosomes are endosome-derived vesicles secreted by multiple cell types, including tumor cells. The vesicles are formed intracellularly by invagination followed by fusion with the multivesicular body (MVB). The MVB merges with the plasma membrane, resulting in the exocytosis of exosomes which have membrane protein composition similar to their cell of origin. These circulating vesicles have been shown to participate in cellular communication by transporting mRNAs, microRNAs (miRs) and proteins to target cells, eliciting biological responses. This study examined the dynamics of exosome biosignatures from prostate cancer (PCa) cell lines. Methods: Exosome-associated RNA and surface membrane protein profiles from the prostate cancer cell line VCaP were analyzed by flow sorting (FACS) and a multiplexed immunoassay. The surface membrane protein composition and RNA content of VCaP exosomes were measured through twenty cell line passages. Results: The surface membrane protein composition of VCaP...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::cd12d57c39ba5406aa56dcb99b06be65 https://doi.org/10.1200/jco.2010.28.15_suppl.e21071 Zobrazit plný text záznamu