Surface Chemistry of Bacteriophage and Laser Ablated Nanoparticle Complexes for Pathogen Detection
| Autor: | Rosemonde Mandeville, David Rioux, Michel Meunier, Nancy Tawil, Edward Sacher |
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| Rok vydání: | 2015 |
| Předmět: |
Heterogeneous sample
Pathogen detection biology Chemistry Nanoparticle Nanotechnology 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology biology.organism_classification Laser Photochemistry 01 natural sciences Dark field microscopy Femtosecond laser ablation 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials law.invention Bacteriophage Colloidal nanoparticles General Energy law Physical and Theoretical Chemistry 0210 nano-technology |
| Zdroj: | The Journal of Physical Chemistry C. 119:14375-14382 |
| ISSN: | 1932-7455 1932-7447 |
| DOI: | 10.1021/acs.jpcc.5b02169 |
| Popis: | We report the synthesis of colloidal nanoparticles, produced by the femtosecond laser ablation of a gold target, and their subsequent use in the formation of S. aureus phage–nanoparticle complexes for biodetection purposes. A detailed X-ray photoelectron spectroscopic analysis shows that the negatively charged nanoparticles that are formed interact with and subsequently internalize into the positively charged bacteriophages. These complexes render possible the specific detection of a single S. aureus bacterium in a heterogeneous sample, using dark field microscopy without complex sample treatment or amplification. |
| Databáze: | OpenAIRE |
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