An oligomer complementary to c-myc mRNA inhibits proliferation of HL-60 promyelocytic cells and induces differentiation

Autor:	J T Holt, R L Redner, Arthur W. Nienhuis
Rok vydání:	1988
Předmět:	Cell division Cell growth Cellular differentiation Cell Cell Biology Biology Oligomer Molecular biology chemistry.chemical_compound medicine.anatomical_structure chemistry Cell culture Gene expression medicine Molecular Biology Intracellular
Zdroj:	Molecular and Cellular Biology. 8:963-973
ISSN:	1098-5549 0270-7306
DOI:	10.1128/mcb.8.2.963
Popis:	To study the role of a nuclear proto-oncogene in the regulation of cell growth and differentiation, we inhibited HL-60 c-myc expression with a complementary antisense oligomer. This oligomer was stable in culture and entered cells, forming an intracellular duplex. Incubation of cells with the anti-myc oligomer decreased the steady-state levels of c-myc protein by 50 to 80%, whereas a control oligomer did not significantly affect the c-myc protein concentration. Direct inhibition of c-myc expression with the anti-myc oligomer was associated with a decreased cell growth rate and an induction of myeloid differentiation. Related antisense oligomers with 2- to 12-base-pair mismatches with c-myc mRNA did not influence HL-60 cells. Thus, the effects of the antisense oligomer exhibited sequence specificity, and furthermore, these effects could be reversed by hybridization competition with another complementary oligomer. Antisense inhibition of a nuclear proto-oncogene apparently bypasses cell surface events in affecting cell proliferation and differentiation.
Databáze:	OpenAIRE
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