PROPERTIES OF MULTINUCLEATED GIANT CELLS IN A NEWIN VITRO MODEL FOR HUMAN GRANULOMA FORMATION

Autor: Norbert Reiling, Dagmar Scheel-Toellner, Johannes Gerdes, Helmut Haas, Kai-Michael Toellner, Ulrike Seitzer, Jürgen Galle, Hans-Dieter Flad
Rok vydání: 1997
Předmět:
Zdroj: The Journal of Pathology. 182:99-105
ISSN: 1096-9896
0022-3417
Popis: Multinucleated giant cells (MGCs) are a key feature of granulomas. They have been studied with respect to the mechanism and regulation of their formation, but the function of these cells still remains elusive. A new method for the in vitro generation of granulomas was developed and characterized in which L3 larvae of Nippostrongylus brasiliensis, as a target for the cellular response, were co-incubated with human mononuclear blood cells. The development of epithelioid cells and MGCs was observed and single isolated MGCs were analysed by the reverse transcriptase polymerase chain reaction method. The presence of tumour necrosis factor alpha (TNFα), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), and inducible nitric oxide synthase (iNOS) transcripts in MGCs was demonstrated. It is proposed that MGCs in the granuloma model may in part represent an active cellular constituent involved in granuloma formation and turnover and in the destruction of the irritant. © 1997 John Wiley & Sons, Ltd.
Databáze: OpenAIRE
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