| Autor: |
Glaucia Morgana de Melo Guedes, Rodrigo Machado Pinheiro, Alyne Soares Freitas, Vinicius Carvalho Pereira, Francisco Ivanilsom Firmiano Gomes, Rossana de Aguiar Cordeiro, José Júlio Costa Sidrim, Marcos Fábio Gadelha Rocha, Debora de Souza Collares Maia Castelo-Branco |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Letters in Applied Microbiology. 76 |
| ISSN: |
1472-765X |
| DOI: |
10.1093/lambio/ovad031 |
| Popis: |
This study aimed to standardize the use of an ex vivo wound model for the evaluation of compounds with antibiofilm activity. The in vitro susceptibility of Staphylococcus aureus ATCC 29213 and Pseudomonas aeruginosa ATCC 27853 to ciprofloxacin and polyhexamethylene biguanide (PHMB) was evaluated in planktonic and biofilm growth. The effects of ciprofloxacin and PHMB on biofilms grown on porcine skin explants were evaluated by colony-forming unit (CFU) counting and confocal microscopy. Minimum inhibitory concentrations (MICs) against S. aureus and P. aeruginosa were, respectively, 0.5 and 0.25 µg mL−1 for ciprofloxacin, and 0.78 and 6.25 µg mL−1 for PHMB. Minimum biofilm eradication concentrations (MBECs) against S. aureus and P. aeruginosa were, respectively, 2 and 8 µg mL−1 for ciprofloxacin, and 12.5 and >25 µg mL−1 for PHMB. Ciprofloxacin reduced (P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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