Discovery of unique single nucleotide polymorphisms in rice in response to high nighttime temperature stress using a hybrid sequencing strategy
| Autor: | Jiang-Lin Liao, Hong-Yu Zhang, Dong-Hui Fu, Chao He, Ying-Jin Huang, Li-Li You, Zhaohai Wang, Jian-Bo Song, Hui-Wen Zhou |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Genetics biology Strain (chemistry) Cellular respiration food and beverages Single-nucleotide polymorphism Plant Science 01 natural sciences 03 medical and health sciences 030104 developmental biology Gene expression biology.protein Prolamin Transcription Factor Gene Agronomy and Crop Science Gene Ecology Evolution Behavior and Systematics 010606 plant biology & botany Peroxidase |
| Zdroj: | Environmental and Experimental Botany. 162:48-57 |
| ISSN: | 0098-8472 |
| Popis: | Global warming-associated increases in temperature, particularly at nighttime, are detrimental to rice grain filling, ultimately leading to losses in grain weight. However, the molecular mechanisms associated with grain weight loss in rice exposed to high nighttime temperature stress are poorly understood. To screen the genes and single nucleotide polymorphisms (SNPs) associated with high nighttime temperature stress in rice, a hybrid sequencing strategy was used to analyze the differentially expressed genes and SNPs between two rice coisogenic strains, a heat-tolerant strain (HTS) and heat-sensitive strain (HSS), following short-term extreme high nighttime temperature stress at the first stage of seed ripening. Ultimately, 56 genes were differentially expressed between HTS and HSS. After short-term extreme high nighttime temperature stress, genes involved in photosynthesis, oxidation, and detoxication by glutathione were upregulated in HSS in comparison to HTS, while that of the heat response-related transcription factor genes were significantly upregulated in HTS in comparison to HSS. Unique SNPs located on the genes peroxidase precursor, glutathione S-transferase GSTU6, glycosyl hydrolases, carboxyvinyl-carboxyphosphonate phosphorylmutase, and prolamin precursor PROLM3 were present in HTS but absent from HSS and showed slight alterations in gene expression between HTS and HSS. The proposed model indicated that high nighttime temperature enhanced cellular respiration, disturbed the oxidant-antioxidant balance, and consumed energy-rich substances, ultimately leading to reduced grain yield in HSS in contrast to HTS. These genes and unique SNPs provide genetic resources for the breeding of heat-tolerant rice varieties, and the model provides insights into the molecular basis of the response of rice to high nighttime temperature stress. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect