Multimodal hyperspectral optical microscopy
| Autor: | Irina V. Novikova, Wayne P. Hess, Patrick Z. El-Khoury, Ashish Bhattarai, Chuck R. Smallwood, Dehong Hu, James Evans, Yu Gong, Leif Hendricks |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Chemical imaging medicine.medical_specialty Microscope business.industry Chemistry General Physics and Astronomy Hyperspectral imaging 02 engineering and technology 021001 nanoscience & nanotechnology Spectral imaging law.invention 03 medical and health sciences 030104 developmental biology Optics Optical microscope law Microscopy medicine Physical and Theoretical Chemistry 0210 nano-technology business Absorption (electromagnetic radiation) Image resolution Remote sensing |
| Zdroj: | Chemical Physics. :25-32 |
| ISSN: | 0301-0104 |
| DOI: | 10.1016/j.chemphys.2017.08.011 |
| Popis: | We describe a unique approach to hyperspectral optical microscopy, herein achieved by coupling a hyperspectral imager to various optical microscopes. Hyperspectral fluorescence micrographs of isolated fluorescent beads are first employed to ensure spectral calibration of our detector and to gauge the attainable spatial resolution of our measurements. Different science applications of our instrument are then described. Spatially over-sampled absorption spectroscopy of a single lipid (18:1 Liss Rhod PE) layer reveals that optical densities on the order of 10−3 can be resolved by spatially averaging the recorded optical signatures. This is followed by three applications in the general areas of plasmonics and bioimaging. Notably, we deploy hyperspectral absorption microscopy to identify and image pigments within a simple biological system, namely, a single live Tisochrysis lutea cell. Overall, this work paves the way for multimodal spectral imaging measurements spanning the realms of several scientific disciplines. |
| Databáze: | OpenAIRE |
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