Contribution of the coelomic epithelial cells specific to the left testis in the chicken embryo

Autor: Youhei Mantani, Miho Nishida, Yuria Umemura, Tetsushi Hirano, Nobuhiko Hoshi, Hidefumi Yoshioka, Hiroshi Kitagawa, Kiichi Minami, Toshifumi Yokoyama, Takuya Omotehara
Rok vydání: 2017
Předmět:
Zdroj: Developmental Dynamics. 246:148-156
ISSN: 1058-8388
DOI: 10.1002/dvdy.24469
Popis: BACKGROUND The left male gonad in the chicken embryo has a thickened cortical layer, but it eventually becomes flattened after the onset of testicular development. Because the destination of the cortical cells migrating from the left gonad remains unclear, we examined this issue herein. RESULTS The testis-inducing gene doublesex- and mab-3-related transcription factor 1 (DMRT1) was detected in a proportion of the columnar and cubic epithelial cells in the cortex of the left testis as well as Sertoli cells in both testes. Interestingly, some of the DMRT1-expressing cortical cells were contiguous with Sertoli cells in the testis cord. Some cortical cells exhibited a vimentin-positive cytoplasm that was elongated all the way to the medulla. In addition, a desmosome-like structure was observed between the elongated cytoplasm in these cells and the adjacent Sertoli cell. After the organ culture, a few cells labeled with a fluorescent dye that stained only the cortical cells at the beginning of the culture were located in the testis cord of the left testis. CONCLUSIONS Some cortical cells expressing DMRT1 were suggested to contribute to the Sertoli cells in the testis cord only after the onset of testicular development and only in the left testis. Developmental Dynamics 246:148-156, 2017. © 2016 Wiley Periodicals, Inc.
Databáze: OpenAIRE
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