The molecular determination of emm genotypes in non-group a beta-hemolytic streptococci isolated from clinical samples
Autor: | Fatih Köksal, Cansu Onlen Guneri, Suna Kizilyildirim |
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Rok vydání: | 2021 |
Předmět: | |
Zdroj: | Journal of Clinical Medicine of Kazakhstan. 18:64-68 |
ISSN: | 2313-1519 1812-2892 |
Popis: | Objective: M protein is an important marker in epidemiological and phylogenetic surveillance of Beta-Hemolytic Streptococci. The aim of this study is to determine emm types and their distribution among the GCS/GGS strains isolated from clinical samples. Material and methods: The study includes 98 β-hemolytic streptococcus strains isolated from clinical samples in Cukurova University/Balcali Hospital. 67 of these isolates, defined as serologic, of which 56 were identified as Streptococcus dysgalactiae subsp. equisimilis, 8 as S. anginosus and 3 as S.equi subsp.zooepedimicus, have been included in the study. These strains were also confirmed by the Vitek 2GP-ID system. These isolates were confirmed by the polymerase chain reaction (PCR) method with primers based on groESL sequences. PCR-sequence analysis method developed by the Centers for Disease Control (CDC) was used for typing according to emm polymorphism. Results: 56/67 (83.6%) isolates in which emm-PCR was determined as positive were typed by sequence analysis. The findings were identified using the CDC database, and the most common type was determined to be stG485 (51.85%), stG643 (12.96%) and stG6 (9.25%). Conclusion: Consequently, GCS/GGS should be treated more seriously in our country as in the whole world. |
Databáze: | OpenAIRE |
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