Expression of Rat Liver Cell Membrane Transporters for Thyroid Hormone inXenopus laevisOocytes1

Autor:	Eric P. Krenning, Roelof Docter, Theo J. Visser, M. E. Everts, Georg Hennemann, Edith C. H. Friesema, Paul G. J. van Stralen
Rok vydání:	1997
Předmět:	Messenger RNA medicine.medical_specialty biology Xenopus Oocyte biology.organism_classification chemistry.chemical_compound Endocrinology medicine.anatomical_structure chemistry Hepatocyte Internal medicine Expression cloning Gene expression medicine Choline Incubation
Zdroj:	Endocrinology. 138:1841-1846
ISSN:	1945-7170 0013-7227
DOI:	10.1210/endo.138.5.5114
Popis:	The present study was conducted to explore the possible use of Xenopus laevis oocytes for the expression cloning of cell membrane transporters for iodothyronines. Injection of stage V–VI X. laevis oocytes with 23 ng Wistar rat liver polyadenylated RNA (mRNA) resulted after 3–4 days in a highly significant increase in [125I]T3 (5 nm) uptake from 6.4 ± 0.8 fmol/oocyte·h in water-injected oocytes to 9.2 ± 0.65 fmol/oocyte·h (mean± sem; n = 19). In contrast,[ 125I]T4 (4 nm) uptake was not significantly stimulated by injection of total liver mRNA. T3 uptake induced by liver mRNA was significantly inhibited by replacement of Na+ in the incubation medium by choline+ or by simultaneous incubation with 1μ m unlabeled T3. In contrast, T3 uptake by water-injected oocytes was not Na+ dependent. Fractionation of liver mRNA on a 6–20% sucrose gradient showed that maximal stimulation of T3 uptake was obtained with mRNA of 0.8–2.1 kilobases (kb). In contrast to unfractionated mRNA, the 0.7- to 2.1-kb fraction also signif...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c961b405d6e53f5e0f8fc51582068576 https://doi.org/10.1210/endo.138.5.5114 Zobrazit plný text záznamu