Evaluation of Micropropagation System ofBacopa monnieriL. in Liquid Culture and Its Effect on Antioxidant Properties
| Autor: | Kencho Wangdi, Indira P. Sarethy |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Pharmacology Biology biology.organism_classification 01 natural sciences law.invention 03 medical and health sciences chemistry.chemical_compound Horticulture 030104 developmental biology Murashige and Skoog medium Erlenmeyer flask Complementary and alternative medicine chemistry Micropropagation law Shoot Botany Doubling time Kinetin Bacopa monnieri 010606 plant biology & botany Explant culture |
| Zdroj: | Journal of Herbs, Spices & Medicinal Plants. 22:69-80 |
| ISSN: | 1540-3580 1049-6475 |
| DOI: | 10.1080/10496475.2015.1020404 |
| Popis: | Nodal explants of Bacopa monnieri were cultured in a solid Murashige and Skoog (MS) medium containing 1 mg L−1 kinetin, transferred after 30 days to a liquid MS medium of the same composition in 100 mL and 250 mL Erlenmeyer flasks (with 10 mL and 50 mL medium, respectively) and Phyta Jars (with 10 mL medium) and subcultured every 7 d. Shoot doubling time was estimated and the total antioxidant capacity of aqueous extracts from liquid-cultured and acclimatized plants was studied. In the solid medium, 5 rooted shoots/explant were produced after ~20 days, with shoot doubling time of 7.99 days; in liquid media, 36–75 rooted shoots were produced with shoot doubling time of 0.91–1.17 days. Aqueous extracts of acclimatized plants showed 10-fold higher total antioxidant capacity compared to in vitro liquid cultured plantlets. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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