Chronic Intermittent Ethanol and Lipopolysaccharide Exposure Differentially Alter Iba-1-Derived Microglia Morphology in the Prelimbic Cortex and Nucleus Accumbens Core
| Autor: | Kaylee N. Hooker, L. J. Chandler, Scofield, J.A. McFaddin, Benjamin M. Siemsen, Justine D. Landin |
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| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
Microglia Chemistry medicine.medical_treatment Infralimbic cortex Nucleus accumbens Cortex (botany) Cell biology 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure Cytokine Neuroimmune system Neuroplasticity medicine Soma 030217 neurology & neurosurgery 030304 developmental biology |
| Popis: | Chronic intermittent ethanol (CIE) exposure in rats induces neuroplastic adaptations in the prelimbic cortex (PL) and nucleus accumbens core (NAcore) during withdrawal, contributing to deficits in decision-making and escalation of alcohol consumption. Increased evidence has linked neuroplastic changes associated with the development of alcohol dependence and withdrawal to neuroimmune signaling and activation of microglia. Microglia exhibit a well-defined structure/function relationship, with alterations in cytokine release occurring concomitantly with reconfiguration of cellular dimensions and redistribution of fine processes. Accordingly, gaining a better understanding of ethanol-induced microglia activation will provide insight into how neuroimmune signaling may impact neural structure and function following ethanol exposure. The present study characterized CIE-induced alterations in microglia morphology in the PL and NAcore using confocal microscopy and 3D image reconstruction. Rats were sacrificed at peak withdrawal, 10 hours after discontinuation of 15 days of CIE or Air exposure. CIE-exposed rats exhibited an increase in microglia soma size in the PL and NAcore compared to Air-exposed control rats. However, the CIE-mediated increase in soma volume in the PL cortex was accompanied by a reduction in microglial complexity. While systemic LPS elicited similar increases in soma volume in the PL cortex and NAcore, the LPS-induced increase in soma size was associated with increased complexity of microglial processes in the PL cortex, an effect opposite to that of CIE. These data are consistent with the hypothesis that the microglial activation state following CIE is structurally and chemically distinct from the activation state associated with induction of the innate neuroimmune system following systemic LPS exposure. |
| Databáze: | OpenAIRE |
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