Functional Viability: Measurement of Synaptic Vesicle Pool Sizes
| Autor: | Jana Katharina Wrosch, Teja W. Groemer |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Fluorescence-lifetime imaging microscopy Chemistry Chemical synapse Cell Neurotransmission Synaptic vesicle Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure medicine Synaptic vesicle recycling Viability assay 030217 neurology & neurosurgery Function (biology) |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493969593 |
| DOI: | 10.1007/978-1-4939-6960-9_15 |
| Popis: | Neurons and their function of conveying information across a chemical synapse are highly regulated systems. Impacts on their functional viability can occur independently from changes in morphology. Here we describe a method to assess the size of synaptic vesicle pools using live cell fluorescence imaging and a genetically encoded probe (pHluorin). Assessing functional parameters such as the size of synaptic vesicle pools can be a valuable addition to common assays of neuronal cell viability as they demonstrate that key cellular functions are intact. |
| Databáze: | OpenAIRE |
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