Evaluation Effects of Estrogen and Isoflavones of Soy on Oxidative Stress in the Adrenal Glands of Rats
| Autor: | Clélia Rejane Antônio Bertoncini, Gui Mi Ko, Michele Longoni Calió, Darci Souza Marinho |
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| Rok vydání: | 2016 |
| Předmět: |
medicine.medical_specialty
Adrenal gland medicine.drug_class medicine.medical_treatment Hormone replacement therapy (menopause) Isoflavones medicine.disease medicine.disease_cause Biochemistry Menopause chemistry.chemical_compound Endocrinology medicine.anatomical_structure chemistry Estrogen Physiology (medical) Internal medicine medicine Ovariectomized rat Phytoestrogens Oxidative stress |
| Zdroj: | Free Radical Biology and Medicine. 100:S104 |
| ISSN: | 0891-5849 |
| Popis: | Hormone replacement therapy is mainly used for relief postmenopausal symptoms as osteoporosis. In consequence of some contraindications of this method, many studies aim the development of alternative therapies. Among them we can highlight the use of phytoestrogens such as isoflavones. In this study we evaluated the proliferative and antioxidant potential of estrogen and isoflavones in the adrenal gland of ovariectomized (Ovx) rats. We used 20 rats of three-months-old, divided into four groups: GI - sham-operated (estrous phase), GII- Ovx with receiving propyleneglycol; GIII - Ovx with receiving isoflavones (150mg/Kg) and GIV – Ovx with receiving 17beta-estrogen (10μg/Kg). All drugs were administered daily and after 30 days of treatment, the animals were euthanized and a part of the adrenal gland was fixed in 4% PFA and processed for immunohistochemistry and the other part was dipped in liquid nitrogen for gene expression analysis by qRT-PCR. The superoxide anion was measured by DHE staining and the histomorphometry was analysed by H.E. Treatment with estrogen showed a largest increase in the layers of the adrenal than isoflavones. These hypertrofic effects agree with the gene expression elevation of Ki67 and VEGF, which did not occur with the caspase 3, indicating that isoflavones have great proliferative effect on the adrenal gland but no protective effect against apoptosis. Also, the group treated with isoflavones had lower levels of superoxide anion compared to the estrogen group, showing that isoflavones can reduce oxidative stress without promoting trophic effect in the adrenal glands of rats. Taken together, our results indicate positively the therapeutic potential of isoflavones and can contribute to the development of effective therapies to decrease the symptoms of menopause. Financial Support: FAPESP and CNPq |
| Databáze: | OpenAIRE |
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