Biological variation and the effect of fasting and halothane anesthesia on plasma glutathione S-transferase concentrations
| Autor: | A F Howie, G B Drummond, L. M. Aldridge, David C. Ray, H J Spens, Geoff J. Beckett |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
chemistry.chemical_classification
medicine.medical_specialty biology Chemistry Biochemistry (medical) Clinical Biochemistry Albumin Glutathione Hemoconcentration chemistry.chemical_compound Endocrinology Glutathione S-transferase Enzyme Internal medicine Blood plasma biology.protein medicine Amylase Quantitative analysis (chemistry) |
| Zdroj: | Clinical Chemistry. 41:668-671 |
| ISSN: | 1530-8561 0009-9147 |
| DOI: | 10.1093/clinchem/41.5.668 |
| Popis: | Using a specific RIA, we have investigated in patients and volunteers whether fasting, diminished hepatic clearance, hemoconcentration, or within-day biological variation might be responsible for the transient increases in plasma glutathione S-transferase (GST) concentration observed after anesthesia. GST concentration was measured in 44 healthy volunteers after an overnight fast and at 3, 6, and 24 h after the fasting sample. The concentration was significantly lower at 3 and 6 h after than in the fasting sample (P = 0.0019 and P = 0.015, respectively). The change in GST concentration caused by fasting was examined in 30 subjects by comparing pre- and postfasting values. Fasting had no significant effect on GST concentration overall (P = 0.4721), but two individuals showed a marked increase in GST concentration after fasting overnight. In a separate study of 10 patients, plasma amylase activity and plasma concentrations of GST and albumin were measured immediately before and 3 h after induction of halothane anesthesia. Although GST concentration was increased at 3 h in each of the 10 patients, plasma amylase activity and plasma albumin concentration were significantly decreased in all patients (P = 0.002). Apparently, increases in GST concentration after anesthesia do not result from incidental factors. |
| Databáze: | OpenAIRE |
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