Genome-wide identification and characterization ofHSPgene superfamily in whitefly (Bemisia tabaci) and expression profiling analysis under temperature stress

Autor: Shu-Sheng Liu, Fei-Xue Ban, Dan-Tong Zhu, Xin-Ru Wang, Chao Wang, Xiao-Wei Wang
Rok vydání: 2017
Předmět:
Zdroj: Insect Science. 26:44-57
ISSN: 1672-9609
DOI: 10.1111/1744-7917.12505
Popis: Heat shock proteins (Hsp) are essential molecular chaperones that play important roles in the stress stimulation of insects. Bemisia tabaci, a phloem feeder and invasive species, can cause extensive crop damage through direct feeding and transmission of plant viruses. Here we employed comprehensive genomics approaches to identity HSP superfamily members in Middle East Asia Minor 1 whitefly genome. In total, we identified 26 Hsp genes, including 3 Hsp90, 17 Hsp70, 1 Hsp60 and 5 sHSP (small heat shock protein, sHSP) genes. The HSP gene superfamily of whitefly is expanded compared with other five insects surveyed here. The gene structures among the same families are relatively conserved. Meanwhile, the motif compositions and secondary structures of BtHsp proteins were predicted. In addition, qPCR analysis showed that the expression patterns of BtHsp gene superfamily were diverse across different tissues of whiteflies. Most Hsp genes were induced or repressed by thermal stress (40°C) and cold treatment (4°C) in whitefly. While silencing the expression of BtHsp70-6 significantly decreased the survival rate of whitefly under 45°C. All the results shown the Hsps conferred thermo-tolerance or cold-tolerance to whiteflies that protect them from being affected by detrimental temperature condition. Our observations highlighted the molecular evolutionary properties and the response mechanism to temperature assaults of Hsp genes in whitefly. This article is protected by copyright. All rights reserved
Databáze: OpenAIRE
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