Dual wavelength stimulation of polymeric nanoparticles for photothermal therapy
| Autor: | Sneha S. Kelkar, Richard Albright, Phil Harrington, Eleanor McCabe-Lankford, Nicole Levi-Polyachenko |
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| Rok vydání: | 2016 |
| Předmět: |
chemistry.chemical_classification
Materials science business.industry Near-infrared spectroscopy Nanoparticle 02 engineering and technology Dermatology Polymer Photothermal therapy Conjugated system 010402 general chemistry 021001 nanoscience & nanotechnology Laser 01 natural sciences 0104 chemical sciences law.invention chemistry law Heat generation Optoelectronics Surgery 0210 nano-technology business Absorption (electromagnetic radiation) |
| Zdroj: | Lasers in Surgery and Medicine. 48:893-902 |
| ISSN: | 0196-8092 |
| DOI: | 10.1002/lsm.22583 |
| Popis: | Background and Objective Photothermal therapy (PTT) has several applications in the areas of wound healing, pain management, bacterial infection control, and cancer treatment dependent on the temperature that is generated. PTT is often used exclusively with near infrared (NIR) light and most nanoparticles (NP) used for PTT are designed to absorb within one narrow range of wavelengths. We have developed a dual-wavelength photothermal therapy by capitalizing on the dual absorption of nanoparticles in the blue and NIR range. Materials and Methods Our lab has previously developed NP based on the semiconducting, conjugated polymer poly[4,4-bis(2-ethylhexyl)-cyclopenta[2,1-b;3,4-b′]dithiophene-2,6-diyl-alt-2,1,3-benzoselenadiazole-4,7-diyl] (PCPDTBSe). The NP have strong absorption in the blue and NIR regions. In this report, we have explored the heat generated by PCPDTBSe NP using simultaneous delivery of 450 and 800 nm light, either independently or together for photothermal ablation of mouse colorectal cancer cells. Results The heat generation studies indicated that the use of either 450 or 800 nm wavelengths at the same fluences produced approximately the same temperature change of deionized water. Fluences of 114.6 and 229.2 J/cm2, utilizing 450 or 800 nm light applied individually resulted in temperatures of 8–47°C above ambient temperature, leading to a 90% reduction in cell viability. Simultaneous stimulation of the PCPDTBSe NP with 450 and 800 nm light effectively doubles the effective power delivered, resulting in temperatures 18–63°C above ambient and 100% photothermal ablation of the colorectal cancer cells. Conclusion The results of this study demonstrate that PCPDTBSe polymer NP can be utilized as effective PTT agents by capitalizing on their dual absorption of both blue and NIR light. Lasers Surg. Med. © 2016 Wiley Periodicals, Inc. |
| Databáze: | OpenAIRE |
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