Survival of mycorrhizal fungal isolates stored in sterile water at two temperatures and retrieved on solid and liquid nutrient media

Autor:	Jane E. Smith, Donaraye McKay, Randy Molina
Rok vydání:	1994
Předmět:	Laccaria food.ingredient biology Sterile water Immunology General Medicine biology.organism_classification Applied Microbiology and Biotechnology Microbiology Solid medium Nutrient Rhizopogon food Botany Genetics Agar Mycorrhiza Molecular Biology
Zdroj:	Canadian Journal of Microbiology. 40:736-742
ISSN:	1480-3275 0008-4166
DOI:	10.1139/m94-117
Popis:	Vegetatively colonized agar cores of 169 reputed mycorrhizal fungal isolates, representing 18 Ascomycotina, 141 Basidiomycotina, and 10 Deuteromycotina fungi, were stored at 4 and 18 °C in tubes of sterile deionized water. Viability, based on growth on solid and liquid nutrient media at 18 °C, was determined after a 20-month storage period. Ninety-five percent of the isolates were retrieved in at least one of three treatments: 89% in liquid nutrient media and 84% on solid media after storage at 18 °C, and 78% on solid media after storage at 4 °C. Nearly all Ascomycotina and Deuteromycotina isolates were retrieved regardless of the storage temperature or form of retrieval medium. Basidiomycotina isolates differed more in survival and percentage of viability than Ascomycotina or Deuteromycotina. For example, storage temperature influenced the viability of Laccaria isolates and the form of retrieval medium influenced the viability of Rhizopogon isolates.Key words: culture maintenance, fungal storage, long-term storage, mycorrhizal fungal isolates, water storage.
Databáze:	OpenAIRE
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