| Autor: |
Xing Shu, Wu Hankui, Ninghui Duan, Xiaoyu Leng, Liu Jianmin, Zhou Mingyang, Ru Han, Liwen Han, Liu Wenjie, Yongchun Zhang, Xinxin Zhang, Xiaobin Li |
| Rok vydání: |
2019 |
| Předmět: |
|
| DOI: |
10.20944/preprints201909.0024.v1 |
| Popis: |
JBIR-99 is a secondary metabolite of marine fungi that has been shown to possess strong antibiotic activity. An efficient approach using a combination of size exclusion chromatography with a Sephadex LH-20 and high-speed counter-current chromatography (HSCCC) has been successfully developed for the isolation and purification of a polyketide from the solid-state fermentation of Meyerozyma guilliermondii. The active compound was isolated with purity >95% by HSCCC using an optimized solvent system composed of petroleum ether–ethyl acetate– 95% ethanol–water (5:3:5:3, v/v/v/v) after size exclusion chromatography. This compound was successfully purified in the quantity of 68 mg from 120 mg of the crude extract. The structure of JBIR-99 was elucidated and assigned by 1D, 2D NMR spectroscopic, and positive HRESITOFMS. Moreover, the relative configuration of compound JBIR-99, displaying a quite complex multi-ring structure, is determined by X-ray crystallography for the first time. The purification method developed for JBIR-99 will facilitate the further investigation and development of this antibiotic agent as a lead compound. Furthermore, it is suggested that the combination of size exclusion chromatography and HSCCC could be more widely applied for the isolation and purification of polyketides from marine fungi. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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