Intrafollicular Concentrations of Steroids and Steroidogenic Enzymes in Relation to Follicular Development in the Mare1
| Autor: | Nadine Gérard, Ghylène Goudet, François Belin, Guy Duchamp |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
endocrine system
medicine.medical_specialty medicine.drug_class Granulosa cell media_common.quotation_subject Biology 03 medical and health sciences 0302 clinical medicine Internal medicine Follicular phase medicine Aromatase Ovulation 030304 developmental biology media_common 0303 health sciences 030219 obstetrics & reproductive medicine Cholesterol side-chain cleavage enzyme Steroidogenic acute regulatory protein Cell Biology General Medicine Follicular fluid Endocrinology Reproductive Medicine biology.protein Gonadotropin |
| Zdroj: | Biology of Reproduction. 62:1335-1343 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod62.5.1335 |
| Popis: | The objective of the present study was to determine the changes in follicular fluid steroid concentrations and in granulosa cell steroidogenic enzyme expression during the follicular phase, in relation to follicular size and physiological status in the mare. Follicular fluid and follicular cells were recovered by ultrasound-guided follicular punctures either around the time of emergence of the dominant follicle, at the end of the dominant follicle growth, or at the preovulatory stage, after injection of gonadotropin to induce ovulation. Cellular relative amounts of steroidogenic acute regulatory protein (StAR), P450-side chain cleavage (P450scc), 3β-hydroxysteroid dehydrogenase (3βHSD), 17α-hydroxylase, and aromatase were assessed by semiquantitative Western blot and densitometry. Follicular fluid was assayed for cholesterol concentrations by colorimetric assay and for progesterone, testosterone, and estradiol-17β concentrations by RIA. Intrafollicular concentrations of progesterone and estradiol... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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