| Autor: |
Feng Gao, Bojia Han, Yin Chen, Fangfang Sun, Jianhui Yang, Chun-Yu Han |
| Rok vydání: |
2022 |
| DOI: |
10.1101/2022.02.22.481407 |
| Popis: |
Argonaute proteins are characterized by highly efficient guide DNA/RNA-directed binding to target nucleic acids with high fidelity. Employing this feature, we designed an argonaute-facilitated PCR platform by making use of an argonaute derived from Mesorhizobium japonicum (MejAgo) which does not carry nuclease activity and exposes the 3’ end of guide DNA when binding. Each reaction cycle of the MejAgo-PCR platform consists of a denaturing step and a polymerase-mediated extension step, omitting the annealing step required by the traditional PCR. More importantly, MejAgo-PCR could significantly improve the sensitivity of PCR in template detection due to the argonaute-facilitated pairing between guide DNA/primer and template. Thus, an argonaute-facilitated PCR has the potential to be developed as an advanced PCR platform with higher sensitivity and efficiency. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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