Specific blockage of the immune response by an excess of Salmonella typhi Vi-antigen

Autor: T. B. Prigozhina
Rok vydání: 1978
Předmět:
Zdroj: Bulletin of Experimental Biology and Medicine. 85:483-486
ISSN: 1573-8221
0007-4888
Popis: Intravenous injection of 200 μg of Vi-antigen into adult mice induces a state of short-term (10–12 days) areactivity in the animals. The observed depression of the immune response is due to blockage of the immunocompetent cells and not to masking of antibody production through binding with the excess of free antigen. Washing a suspension of spleen cells twice before using them in the local passive hemolysis in gel test did not reduce the blockage of the immune response; moreover, no free antigen capable of binding antibodies produced by cells of the immune animal could be found in the spleen of the experimental animals. Blockage of the immune response could be abolished by injecting heterologous antiserum against Vi-antigen into the animals 18–24 h before Jerne's test. Injection of 6-thioguanine after 200 μg of Vi-antigen prevented restoration of the immune response by means of the antiserum. It is concluded from the results that injection of a massive dose of Vi-antigen does not block proliferation and differentiation of antigen-recognizing cells but inhibits the synthesis or secretion of antibodies.
Databáze: OpenAIRE
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