Nutrient concentration-sensitive microorganism-based biosensor
| Autor: | Michael J. Schöning, Tatsuo Yoshinobu, Michael Keusgen, Marcus E. M. Baumann, Torsten Wagner, Carl Frederik Werner, Christoph Krumbe, Thorsten Selmer, Simone Groebel |
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| Rok vydání: | 2012 |
| Předmět: |
Chromatography
biology Chemistry Microorganism Surfaces and Interfaces Light-addressable potentiometric sensor Condensed Matter Physics biology.organism_classification medicine.disease_cause Surfaces Coatings and Films Electronic Optical and Magnetic Materials Nutrient Biochemistry Materials Chemistry medicine Potentiometric sensor Electrical and Electronic Engineering Extracellular acidification Escherichia coli Biosensor Bacteria |
| Zdroj: | physica status solidi (a). 209:900-904 |
| ISSN: | 1862-6300 |
| DOI: | 10.1002/pssa.201100801 |
| Popis: | This work presents a biosensor based on living cells immobilised on a light-addressable potentiometric sensor (LAPS). The set-up is sensitive to the concentrations of nutrients metabolised by bacteria, which results in an extracellular acidification. This is exemplarily demonstrated with glucose as nutrient and Escherichia coli as microorganism. The function of this biosensor is comparable to an enzyme-based field-effect sensor when using diffusion limitation. With the help of addressability of the LAPS an on-chip differential set-up was developed. Thus, external influences such as sensor drift, temperature and external pH changes can be minimised. |
| Databáze: | OpenAIRE |
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