Chitinophaga extrema sp. nov., isolated from subsurface soil and leaf litter in a tropical peat swamp forest
| Autor: | Larry Croft, Joash Ban Lee Tan, Stella Loke, Li Wen Wong, Pooria Pasbakhsh, Sui Mae Lee, Heera Rajandas, Calvin Bok Sun Goh, Catherine M. Yule, Sivachandran Parimannan |
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| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 70:6355-6363 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijsem.0.004539 |
| Popis: | A Gram-negative, filamentous aerobic bacterium designated as strain Mgbs1T was isolated on 12 April 2017 from the subsurface soil and leaf litter substrate at the base of a Koompassia malaccensis tree in a tropical peat swamp forest in the northern regions of the state of Selangor, Malaysia (3° 39′ 04.7′ N 101° 17′ 43.7′′ E). Phylogenetic analyses based on the full 16S rRNA sequence revealed that strain Mgbs1T belongs to the genus Chitinophaga with the greatest sequence similarity to Chitinophaga terrae KP01T (97.65 %), Chitinophaga jiangningensis DSM27406T (97.58 %), and Chitinophaga dinghuensis DHOC24T (97.17 %). The major fatty acids of strain Mgbs1T (>10 %) are iso-C15 : 0, C16 : 1 ω5c and iso-C17 : 0 3-OH while the predominant respiratory quinone is menaquinone-7. Strain Mgbs1T has a complete genome size of 8.03 Mb, with a G+C content of 48.5 mol%. The DNA–DNA hybridization (DDH) score between strain Mgbs1T and C. jiangningensis DSM27406T was 15.9 %, while in silico DDH values of strain Mgbs1T against C. dinghuensis DHOC24T and C. terrae KP01T were 20.0 and 19.10% respectively. Concurrently, Average Nucleotide Identity (ANI) scores between strain Mgbs1T against all three reference strains are 73.2 %. Based on the phenotypic, chemotaxonomic, and phylogenetic consensus, strain Mgbs1T represents a novel species of the genus Chitinophaga , for which the name Chitinophaga extrema sp. nov. is proposed (=DSM 108835T=JCM 33276T). |
| Databáze: | OpenAIRE |
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