Identification of the coding region for the putidaredoxin reductase gene from the plasmid of Pseudomonas putida

Autor:	Irwin C. Gunsalus, Hideo Koga, Naoko Moriwaki, Charles Romeo, Kerry T. Yasunobu
Rok vydání:	1987
Předmět:	chemistry.chemical_classification Methionine Biology Reductase biology.organism_classification Biochemistry Molecular biology Pseudomonas putida Amino acid chemistry.chemical_compound Plasmid chemistry bacteria Coding region Peptide sequence Gene
Zdroj:	Journal of Protein Chemistry. 6:253-261
ISSN:	1573-4943 0277-8033
DOI:	10.1007/bf00250289
Popis:	The first 12 NH2-terminal amino acids of the Pseudomonas putida putidaredoxin reductase were shown to be Met-Asn-Ala-Asn-Asp-Asn-Val-Val-Ile-Val-Gly-Thr. Comparison of these data with the DNA sequence of the BamHI-HindIII 197-base fragment derived from the PstI 2.2-kb fragment obtained from the P. putida plasmid showed that the putidaredoxin reductase gene was downstream from the cytochrome P-450 gene and the intergenic region had the 24-nucleotide sequence TAAACACATGGGAGTGCGTGCTAA. The Shine-Dalgarno sequence GGAG was detected in this region. The initiating triplet for the reductase gene was GTG, which normally codes for valine, but in the initiating codon position codes for methionine. From the amino acid sequence and X-ray data comparisons with other flavoproteins, what appears to be the AMP binding region of the FAD can be recognized in the NH2-terminal portion of the reductase involving residues 5–35.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c4cb1f2c47ebc47f7777ec371a0cb14a https://doi.org/10.1007/bf00250289 Zobrazit plný text záznamu Full text from SpringerLink